Correction of multi-gene deficiency in vivo using a single ‘self-cleaving’ 2A peptide-based retroviral vector.

Attempts to generate reliable and versatile vectors for gene therapy and biomedical research that express multiple genes have met with limited success. Here we used Picornavirus ‘self-cleaving’ 2A peptides, or 2A-like sequences from other viruses, to generate multicistronic retroviral vectors with efficient translation of four cistrons.

Adenovirus Antibody
abx021512-1mg	Abbexa	1 mg	EUR 710.4

Adenovirus Antibody
abx021521-02mg	Abbexa	0.2 mg	EUR 1345.2

Adenovirus Antibody
abx021524-1mg	Abbexa	1 mg	EUR 1220.4

Adenovirus Antibody
abx022913-1ml	Abbexa	1 ml	EUR 718.8

Adenovirus antibody
10-3147	Fitzgerald	1 mg	EUR 336
Description: Mouse Monoclonal Adenovirus antibody

Adenovirus antibody
10-3148	Fitzgerald	1 mg	EUR 336
Description: Mouse Monoclonal Adenovirus antibody

Adenovirus antibody
10-A02A	Fitzgerald	200 ug	EUR 195
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10-A02B	Fitzgerald	200 ug	EUR 205
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10-A02C	Fitzgerald	200 ug	EUR 473
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10-A02E	Fitzgerald	1 mg	EUR 463
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10-A02F	Fitzgerald	200 ug	EUR 375
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10-A02G	Fitzgerald	200 ug	EUR 195
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10-A02H	Fitzgerald	200 ug	EUR 205
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10R-A115a	Fitzgerald	1 mg	EUR 339
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10R-A115b	Fitzgerald	1 mg	EUR 425
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10R-A115c	Fitzgerald	1 mg	EUR 400
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
10C-CR1238M2	Fitzgerald	100 ug	EUR 205
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody
20-AG02	Fitzgerald	1 mg	EUR 110
Description: Goat polyclonal Adenovirus antibody

Adenovirus antibody
70R-AR025	Fitzgerald	100 ug	EUR 280
Description: Affinity purified Rabbit polyclonal Adenovirus antibody

Adenovirus, Antibody
GWB-39B98C	GenWay Biotech	1 mg	Ask for price

Adenovirus, Antibody
GWB-510390	GenWay Biotech	0.1 mg	Ask for price

Adenovirus, Antibody
GWB-2E1DF0	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody
GWB-2E359C	GenWay Biotech	0.5 ml	Ask for price

Adenovirus Antibody
GWB-14B840	GenWay Biotech	1 mg	Ask for price

Adenovirus, Antibody
GWB-8095EF	GenWay Biotech	0.2 mg	Ask for price

Adenovirus, Antibody
GWB-8963D6	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody
GWB-8B7471	GenWay Biotech	1 ml	Ask for price

Adenovirus, Antibody
GWB-8FD899	GenWay Biotech	0.2 mg	Ask for price

Adenovirus Antibody
GWB-9B3B15	GenWay Biotech	0.5 mg	Ask for price

Adenovirus Antibody
GWB-9EE8AF	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody
GWB-67783A	GenWay Biotech	1 ml	Ask for price

Adenovirus Antibody
GWB-73B6A4	GenWay Biotech	1 ml	Ask for price

Adenovirus Antibody
GWB-7F2745	GenWay Biotech	1 mg	Ask for price

Adenovirus, Antibody
GWB-F2B569	GenWay Biotech	0.2 mg	Ask for price

Adenovirus, Antibody
GWB-F60805	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody
GWB-F940B1	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody
GWB-B75AC1	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody
GWB-BA59D4	GenWay Biotech	0.05 mg	Ask for price

Adenovirus, Antibody
GWB-D68464	GenWay Biotech	0.2 mg	Ask for price

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GWB-D96967	GenWay Biotech	1 ml	Ask for price

Adenovirus Antibody
MBS5680034-1mg	MyBiosource	1mg	EUR 485

Adenovirus Antibody
MBS5680034-5x1mg	MyBiosource	5x1mg	EUR 1980

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MBS568023-1mg	MyBiosource	1mg	EUR 485

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MBS568023-5x1mg	MyBiosource	5x1mg	EUR 1980

Adenovirus Antibody
MBS568234-1mg	MyBiosource	1mg	EUR 485

Adenovirus Antibody
MBS568234-5x1mg	MyBiosource	5x1mg	EUR 1980

Adenovirus Antibody
MBS568252-1mg	MyBiosource	1mg	EUR 485

Adenovirus Antibody
MBS568252-5x1mg	MyBiosource	5x1mg	EUR 1980

Adenovirus Antibody
MBS568324-1mg	MyBiosource	1mg	EUR 365

Adenovirus Antibody
MBS568324-5x1mg	MyBiosource	5x1mg	EUR 1435

Adenovirus Antibody
MBS568388-1mg	MyBiosource	1mg	EUR 365

Adenovirus Antibody
MBS568388-5x1mg	MyBiosource	5x1mg	EUR 1435

Adenovirus Antibody
MBS568550-05mL	MyBiosource	0.5mL	EUR 300

Adenovirus Antibody
MBS568550-5x05mL	MyBiosource	5x0.5mL	EUR 1160

Adenovirus Antibody
MBS569112-1mg	MyBiosource	1mg	EUR 440

Adenovirus Antibody
MBS569112-5x1mg	MyBiosource	5x1mg	EUR 1790

Adenovirus Antibody
MBS569113-1mg	MyBiosource	1mg	EUR 440

Adenovirus Antibody
MBS569113-5x1mg	MyBiosource	5x1mg	EUR 1790

Adenovirus Antibody
MBS569114-1mg	MyBiosource	1mg	EUR 440

Adenovirus Antibody
MBS569114-5x1mg	MyBiosource	5x1mg	EUR 1790

Adenovirus Antibody
MBS569115-1mg	MyBiosource	1mg	EUR 440

Adenovirus Antibody
MBS569115-5x1mg	MyBiosource	5x1mg	EUR 1790

Adenovirus Antibody
MBS569813-1mg	MyBiosource	1mg	EUR 650

Adenovirus Antibody
MBS569813-5x1mg	MyBiosource	5x1mg	EUR 2735

Adenovirus antibody
MBS831119-1mg	MyBiosource	1mg	EUR 565

Adenovirus antibody
MBS831119-5x1mg	MyBiosource	5x1mg	EUR 2385

Adenovirus antibody
MBS531441-02mg	MyBiosource	0.2mg	EUR 310

Adenovirus antibody
MBS531441-5x02mg	MyBiosource	5x0.2mg	EUR 1250

Adenovirus antibody
MBS531639-1mg	MyBiosource	1mg	EUR 575

Adenovirus antibody
MBS531639-5x1mg	MyBiosource	5x1mg	EUR 2440

Adenovirus antibody
MBS531712-1mg	MyBiosource	1mg	EUR 620

Adenovirus antibody
MBS531712-5x1mg	MyBiosource	5x1mg	EUR 2645

Adenovirus antibody
MBS531722-05mg	MyBiosource	0.5mg	EUR 505

Adenovirus antibody
MBS531722-1mg	MyBiosource	1mg	EUR 935

Adenovirus antibody
MBS531722-5x1mg	MyBiosource	5x1mg	EUR 4065

Adenovirus antibody
MBS531797-1mg	MyBiosource	1mg	EUR 610

Adenovirus antibody
MBS531797-5x1mg	MyBiosource	5x1mg	EUR 2595

Adenovirus antibody
MBS531815-05mg	MyBiosource	0.5mg	EUR 665

Adenovirus antibody
MBS531815-5x05mg	MyBiosource	5x0.5mg	EUR 2840

Adenovirus antibody
MBS531918-02mg	MyBiosource	0.2mg	EUR 310

Adenovirus antibody
MBS531918-5x02mg	MyBiosource	5x0.2mg	EUR 1250

Adenovirus antibody
MBS532750-1mg	MyBiosource	1mg	EUR 565

Adenovirus antibody
MBS532750-5x1mg	MyBiosource	5x1mg	EUR 2385

Adenovirus antibody
MBS530168-02mg	MyBiosource	0.2mg	EUR 230

Adenovirus antibody
MBS530168-5x02mg	MyBiosource	5x0.2mg	EUR 875

Adenovirus antibody
MBS530357-1mg	MyBiosource	1mg	EUR 610

Adenovirus antibody
MBS530357-5x1mg	MyBiosource	5x1mg	EUR 2590

Adenovirus antibody
MBS530464-02mg	MyBiosource	0.2mg	EUR 310

Adenovirus antibody
MBS530464-5x02mg	MyBiosource	5x0.2mg	EUR 1250

Adenovirus antibody
MBS530700-02mg	MyBiosource	0.2mg	EUR 305

Adenovirus antibody
MBS530700-5x02mg	MyBiosource	5x0.2mg	EUR 1215

Adenovirus antibody
MBS535174-INQUIRE	MyBiosource	INQUIRE	Ask for price

Adenovirus antibody
MBS535992-01mg	MyBiosource	0.1mg	EUR 480

Adenovirus antibody
MBS535992-5x01mg	MyBiosource	5x0.1mg	EUR 2010

Adenovirus antibody (HRP)
60-A02	Fitzgerald	1 ml	EUR 420
Description: Goat polyclonal Adenovirus antibody (HRP) conjugated

Adenovirus antibody (HRP)
MBS534868-1mL	MyBiosource	1mL	EUR 600

Adenovirus antibody (HRP)
MBS534868-5x1mL	MyBiosource	5x1mL	EUR 2555

Adenovirus Antibody (FITC)
abx021508-1mg	Abbexa	1 mg	EUR 1496.4

Adenovirus Antibody (FITC)
abx022914-1ml	Abbexa	1 ml	EUR 777.6

Adenovirus antibody (FITC)
60C-CR1238GF	Fitzgerald	1 ml	EUR 385
Description: Goat polyclonal Adenovirus antibody (FITC) conjugated

Adenovirus antibody (FITC)
61-A02A	Fitzgerald	100 ug	EUR 275
Description: Mouse monoclonal Adenovirus antibody (FITC)

Adenovirus antibody (FITC)
60-A01	Fitzgerald	1 ml	EUR 385
Description: Goat polyclonal Adenovirus antibody (FITC) conjugated

Adenovirus antibody (FITC)
MBS530396-01mg	MyBiosource	0.1mg	EUR 690

Adenovirus antibody (FITC)
MBS530396-5x01mg	MyBiosource	5x0.1mg	EUR 2950

Adenovirus antibody (FITC)
MBS534377-1mL	MyBiosource	1mL	EUR 560

Adenovirus antibody (FITC)
MBS534377-5x1mL	MyBiosource	5x1mL	EUR 2370

Adenovirus antibody (FITC)
MBS535189-1mL	MyBiosource	1mL	EUR 560

Adenovirus antibody (FITC)
MBS535189-5x1mL	MyBiosource	5x1mL	EUR 2370

Adenovirus Antibody (hexon)
GWB-228B66	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody (hexon)
GWB-3298D7	GenWay Biotech	0.1 mg	Ask for price

Adenovirus Antibody (hexon)
GWB-T00140	GenWay Biotech	0.2 mg	Ask for price

Adenovirus Antibody (hexon)
GWB-T00412	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody (hexon)
GWB-T00505	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody (hexon)
GWB-877511	GenWay Biotech	1 mg	Ask for price

Adenovirus Antibody (hexon)
GWB-DB9D3D	GenWay Biotech	1 mg	Ask for price

Adenovirus antibody (biotin)
60C-CR1238GB	Fitzgerald	1 ml	EUR 385
Description: Goat polyclonal Adenovirus antibody (biotin) conjugated

Adenovirus Antibody (Biotin)
GWB-2DFD26	GenWay Biotech	1 ml	Ask for price

Adenovirus antibody (biotin)
MBS535286-1mL	MyBiosource	1mL	EUR 560

Adenovirus antibody (biotin)
MBS535286-5x1mL	MyBiosource	5x1mL	EUR 2370

Adenovirus 40 & 41 Antibody
abx021513-1mg	Abbexa	1 mg	EUR 710.4

Adenovirus Antibody (40 & 41)
GWB-75C7AA	GenWay Biotech	1 mg	Ask for price

Adenovirus 40 & 41 Antibody
abx021513-400l	Abbexa	400 µl	Ask for price

Adenovirus 40 & 41 Antibody
abx021513-80l	Abbexa	80 µl	EUR 637.5

Adenovirus E1A antibody
20-ES02	Fitzgerald	100 ug	EUR 175
Description: Sheep polyclonal Adenovirus E1A antibody

Adenovirus (ADV) Antibody
abx411041-1ml	Abbexa	1 ml	EUR 610.8

Adenovirus (ADV) Antibody
abx414317-1ml	Abbexa	1 ml	EUR 794.4

Adenovirus (ADV) Antibody
abx414953-1mg	Abbexa	1 mg	EUR 994.8

Adenovirus (ADV) Antibody
abx022913-400l	Abbexa	400 µl	Ask for price

Adenovirus (ADV) Antibody
abx022913-80l	Abbexa	80 µl	EUR 975

Adenovirus (ADV) Antibody
abx021507-400l	Abbexa	400 µl	Ask for price

Adenovirus (ADV) Antibody
abx021507-80l	Abbexa	80 µl	EUR 937.5

Adenovirus (ADV) Antibody
abx021509-400l	Abbexa	400 µl	Ask for price

Adenovirus (ADV) Antibody
abx021509-80l	Abbexa	80 µl	EUR 1150

Adenovirus (ADV) Antibody
abx021510-400l	Abbexa	400 µl	Ask for price

Adenovirus (ADV) Antibody
abx021510-80l	Abbexa	80 µl	EUR 700

Adenovirus (ADV) Antibody
abx021511-400l	Abbexa	400 µl	Ask for price

Adenovirus (ADV) Antibody
abx021511-80l	Abbexa	80 µl	EUR 637.5

Adenovirus (ADV) Antibody
abx021512-400l	Abbexa	400 µl	Ask for price

Adenovirus (ADV) Antibody
abx021512-80l	Abbexa	80 µl	EUR 700

Adenovirus (ADV) Antibody
abx021524-400l	Abbexa	400 µl	Ask for price

Adenovirus (ADV) Antibody
abx021524-80l	Abbexa	80 µl	EUR 1200

Adenovirus (ADV) Antibody
abx414953-200l	Abbexa	200 µl	EUR 775

Adenovirus (ADV) Antibody
abx411041-250g	Abbexa	250 µg	EUR 425

Adenovirus E1A antibody
MBS535047-01mg	MyBiosource	0.1mg	EUR 325

Adenovirus E1A antibody
MBS535047-5x01mg	MyBiosource	5x0.1mg	EUR 1305

Adenovirus -FITC, Antibody
GWB-DD2762	GenWay Biotech	0.1 mg	Ask for price

Adenovirus Hexon Antibody
abx120000-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx120001-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx120002-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx120003-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx120004-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx120005-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx120006-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx120007-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx021514-1mg	Abbexa	1 mg	EUR 1011.6

Adenovirus Hexon Antibody
abx021516-1mg	Abbexa	1 mg	EUR 1011.6

Adenovirus Hexon Antibody
abx021518-1mg	Abbexa	1 mg	EUR 1220.4

Adenovirus Hexon Antibody
abx021519-1mg	Abbexa	1 mg	EUR 1111.2

Adenovirus Hexon Antibody
abx021520-1mg	Abbexa	1 mg	EUR 878.4

Adenovirus Hexon Antibody
abx021523-1mg	Abbexa	1 mg	EUR 878.4

Adenovirus Hexon Antibody
abx021525-1mg	Abbexa	1 mg	EUR 878.4

Adenovirus Hexon Antibody
abx023633-1mg	Abbexa	1 mg	EUR 794.4

Adenovirus Hexon Antibody
abx023661-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx023662-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx023854-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx023856-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx023857-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx023858-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx023872-1mg	Abbexa	1 mg	EUR 718.8

Adenovirus Hexon Antibody
abx024001-1mg	Abbexa	1 mg	EUR 1212

Adenovirus Hexon Antibody
abx024002-1mg	Abbexa	1 mg	EUR 1212

Adenovirus Hexon Antibody
abx024003-1mg	Abbexa	1 mg	EUR 1212

Adenovirus Hexon Antibody
GWB-58B6E6	GenWay Biotech	0.2 mg	Ask for price

Adenovirus Hexon Antibody
GWB-87A6E0	GenWay Biotech	0.2 mg	Ask for price

Adenovirus Hexon Antibody
GWB-A81C1E	GenWay Biotech	0.2 mg	Ask for price

Adenovirus Hexon Antibody
GWB-C965A0	GenWay Biotech	0.2 mg	Ask for price

Adenovirus Hexon Antibody
abx120000-100l	Abbexa	100 µl	Ask for price

Adenovirus Hexon Antibody
abx120000-20l	Abbexa	20 µl	EUR 725

Adenovirus Hexon Antibody
abx120000-50l	Abbexa	50 µl	Ask for price

Adenovirus Hexon Antibody
abx120001-100l	Abbexa	100 µl	Ask for price

Adenovirus Hexon Antibody
abx120001-20l	Abbexa	20 µl	EUR 725

Adenovirus Hexon Antibody
abx120001-50l	Abbexa	50 µl	Ask for price

Adenovirus Hexon Antibody
abx120002-100l	Abbexa	100 µl	Ask for price

Adenovirus Hexon Antibody
abx120002-20l	Abbexa	20 µl	EUR 725

Adenovirus Hexon Antibody
abx120002-50l	Abbexa	50 µl	Ask for price

Adenovirus Hexon Antibody
abx120003-100l	Abbexa	100 µl	Ask for price

Adenovirus Hexon Antibody
abx120003-20l	Abbexa	20 µl	EUR 725

Adenovirus Hexon Antibody
abx120003-50l	Abbexa	50 µl	Ask for price

Adenovirus Hexon Antibody
abx120004-100l	Abbexa	100 µl	Ask for price

Adenovirus Hexon Antibody
abx120004-20l	Abbexa	20 µl	EUR 725

Using the T-cell receptor:CD3 complex as a test system, we show that a single 2A peptide-linked retroviral vector can be used to generate all four CD3 proteins (CD3epsilon, gamma, delta, zeta), and restore T-cell development and function in CD3-deficient mice.

We also show complete 2A peptide-mediated ‘cleavage’ and stoichiometric production of two fluorescent proteins using a fluorescence resonance energy transfer-based system in multiple cell types including blood, thymus, spleen, bone marrow and early stem cell progenitors.