Correction of multi-gene deficiency in vivo using a single ‘self-cleaving’ 2A peptide-based retroviral vector.

Attempts to generate reliable and versatile vectors for gene therapy and biomedical research that express multiple genes have met with limited success. Here we used Picornavirus ‘self-cleaving’ 2A peptides, or 2A-like sequences from other viruses, to generate multicistronic retroviral vectors with efficient translation of four cistrons.

Adenovirus Antibody

abx021512-1mg 1 mg
EUR 592

Adenovirus Antibody

abx021521-02mg 0.2 mg
EUR 1121

Adenovirus Antibody

abx021524-1mg 1 mg
EUR 1017

Adenovirus Antibody

abx022913-1ml 1 ml
EUR 599

Adenovirus antibody

10-3147 1 mg
EUR 359
Description: Mouse Monoclonal Adenovirus antibody

Adenovirus antibody

10-3148 1 mg
EUR 359
Description: Mouse Monoclonal Adenovirus antibody

Adenovirus antibody

10-A02A 200 ug
EUR 354
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10-A02B 200 ug
EUR 281
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10-A02C 200 ug
EUR 228
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10-A02E 1 mg
EUR 530
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10-A02F 200 ug
EUR 224
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10-A02G 200 ug
EUR 354
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10-A02H 200 ug
EUR 402
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10C-CR1238M2 100 ug
EUR 247
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10R-A115a 1 mg
EUR 408
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10R-A115b 1 mg
EUR 489
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

10R-A115c 1 mg
EUR 448
Description: Mouse monoclonal Adenovirus antibody

Adenovirus antibody

20-AG02 1 mg
EUR 116
Description: Goat polyclonal Adenovirus antibody

Adenovirus antibody

70R-AR025 100 ug
EUR 300
Description: Affinity purified Rabbit polyclonal Adenovirus antibody

Anti-Adenovirus antibody

STJ16100464 1 mL
EUR 960

Anti-Adenovirus antibody

STJ180409 0.1 ml
EUR 259

Adenovirus Hexon Antibody

abx120000-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx120001-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx120002-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx120003-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx120004-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx120005-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx120006-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx120007-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx023872-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx024001-1mg 1 mg
EUR 1010

Adenovirus Hexon Antibody

abx024002-1mg 1 mg
EUR 1010

Adenovirus Hexon Antibody

abx024003-1mg 1 mg
EUR 1010

Adenovirus Antibody (FITC)

abx021508-1mg 1 mg
EUR 1247

Adenovirus Hexon Antibody

abx021514-1mg 1 mg
EUR 843

Adenovirus Hexon Antibody

abx021516-1mg 1 mg
EUR 843

Adenovirus Hexon Antibody

abx021518-1mg 1 mg
EUR 1017

Adenovirus Hexon Antibody

abx021519-1mg 1 mg
EUR 926

Adenovirus Hexon Antibody

abx021520-1mg 1 mg
EUR 732

Adenovirus Hexon Antibody

abx021523-1mg 1 mg
EUR 732

Adenovirus Hexon Antibody

abx021525-1mg 1 mg
EUR 732

Adenovirus Antibody (FITC)

abx022914-1ml 1 ml
EUR 648

Adenovirus Hexon Antibody

abx023633-1mg 1 mg
EUR 662

Adenovirus Hexon Antibody

abx023661-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx023662-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx023854-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx023856-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx023857-1mg 1 mg
EUR 599

Adenovirus Hexon Antibody

abx023858-1mg 1 mg
EUR 599

Adenovirus (ADV) Antibody

abx411041-1ml 1 ml
EUR 509

Adenovirus (ADV) Antibody

abx414317-1ml 1 ml
EUR 662

Adenovirus (ADV) Antibody

abx414953-1mg 1 mg
EUR 829

Adenovirus E1A antibody

20-ES02 100 ug
EUR 219
Description: Sheep polyclonal Adenovirus E1A antibody

Adenovirus antibody (FITC)

61-A02A 100 ug
EUR 358
Description: Mouse monoclonal Adenovirus antibody (FITC)

Adenovirus antibody (FITC)

60-A01 1 ml
EUR 367
Description: Goat polyclonal Adenovirus antibody (FITC) conjugated

Adenovirus antibody (HRP)

60-A02 1 ml
EUR 412
Description: Goat polyclonal Adenovirus antibody (HRP) conjugated

Adenovirus antibody (biotin)

60C-CR1238GB 1 ml
EUR 363
Description: Goat polyclonal Adenovirus antibody (biotin) conjugated

Adenovirus antibody (FITC)

60C-CR1238GF 1 ml
EUR 363
Description: Goat polyclonal Adenovirus antibody (FITC) conjugated

Adenovirus protein

30R-AA006X 100 ug
EUR 795
Description: Purified native Adenovirus protein

Mouse antibody for Adenovirus

1433 100 ug
EUR 359.94
Description: This is Mouse monoclonal FITC conjugated antibody against Adenovirus hexon for WB, ELISA.

Adenovirus 40 & 41 Antibody

abx021513-1mg 1 mg
EUR 592

Adenovirus Hexon Antibody (FITC)

abx021515-100ug 100 ug
EUR 739

Adenovirus (ADV) Antibody (Biotin)

abx411042-1ml 1 ml
EUR 592

Adenovirus Hexon Protein Antibody

abx411106-02mg 0.2 mg
EUR 565

Adenovirus Hexon Protein Antibody

abx411107-02mg 0.2 mg
EUR 565

Adenovirus Hexon Protein Antibody

abx411108-02mg 0.2 mg
EUR 565

Adenovirus Hexon Protein Antibody

abx411109-02mg 0.2 mg
EUR 565

Mouse Anti-Adenovirus Monoclonal Antibody

DMAB2923-05mg 0.5 mg
EUR 780

Mouse Anti-Adenovirus Monoclonal Antibody

DMAB2923-1mg 1 mg
EUR 1099

Mouse Anti-Adenovirus Monoclonal Antibody

DMAB2924-05mg 0.5 mg
EUR 897

Mouse Anti-Adenovirus Monoclonal Antibody

DMAB2924-1mg 1 mg
EUR 1339

Mouse Anti-Adenovirus Monoclonal Antibody

DMAB2926 1 mg
EUR 689

Mouse Anti-Adenovirus Monoclonal Antibody

DMAB2929 1 mg
EUR 689

Mouse Anti Adenovirus Monoclonal Antibody

DMABT-51065MA 1 ml
EUR 710

Mouse Anti Adenovirus Monoclonal Antibody

DMABT-51066MA 1 mg
EUR 767

Goat Anti-Adenovirus Polyclonal Antibody

DPAB0136 1 ml
EUR 781

Goat Anti-Adenovirus Polyclonal Antibody

DPAB0137 1 ml
EUR 741

Goat Anti Adenovirus Polyclonal Antibody

DPBT-66803GA 1 ml
EUR 767

Adenovirus-9 E4 Orf1 Antibody

24734-100ul 100ul
EUR 390

Mouse antibody for Adenovirus hexon

1431 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Adenovirus hexon for WB, ELISA.

Mouse antibody for Adenovirus hexon

1481 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Adenovirus hexon for WB, ELISA.

Mouse antibody for Adenovirus hexon

1491 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Adenovirus hexon for WB, ELISA.

Adenovirus Hexon Protein Antibody (FITC)

abx415758-01mg 0.1 mg
EUR 606

Adenovirus-9 E4 Orf1 Antibody

4749-002mg 0.02 mg
EUR 171.82
Description: Adenovirus-9 E4 Orf1 Antibody: The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas. In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4). The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1. It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.

Adenovirus-9 E4 Orf1 Antibody

4749-01mg 0.1 mg
EUR 436.42
Description: Adenovirus-9 E4 Orf1 Antibody: The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas. In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4). The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1. It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.

Adenovirus PCR kit

PCR-H501-48D 50T
EUR 453

Adenovirus PCR kit

PCR-H501-96D 100T
EUR 572

?-Galalactosidase Recombinant Adenovirus

ADV-002 50 ?L
EUR 891
Description: Premade recombinant adenovirus containing the beta-galactosidase gene

Rac1 Recombinant Adenovirus

ADV-149 50 ?L
EUR 891
Description: Premade recombinant adenovirus containing the Rac1 gene

Cdc42 Recombinant Adenovirus

ADV-152 50 ?L
EUR 891
Description: Premade recombinant adenovirus containing the Cdc42 gene

RFP expression Adenovirus

AVP001 1x109 IFU/ml x 200ul
EUR 349
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus

AVP002 1x109 IFU/ml x 200ul
EUR 349
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

Luciferase / GFP Adenovirus

AVP004 1x109 IFU/ml x 200ul
EUR 349
Description: pre-made Luciferase / GFP expression adenovirus, provided in DMEM medium.

Luciferase / RFP Adenovirus

AVP005 1x109 IFU/ml x 200ul
EUR 349
Description: pre-made Luciferase / RFP expression adenovirus, provided in DMEM medium.

CRISPR / hCas9 Adenovirus

AVP010 1x109 IFU/ml x 200ul
EUR 451
Description: pre-made adenovirus expresses the neclear penetrated, human codon optimized wild-type Cas9 endonuclease, provided in DMEM medium.

GFP expression Adenovirus

AVP011 1x109 IFU/ml x 200ul
EUR 349
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

Using the T-cell receptor:CD3 complex as a test system, we show that a single 2A peptide-linked retroviral vector can be used to generate all four CD3 proteins (CD3epsilon, gamma, delta, zeta), and restore T-cell development and function in CD3-deficient mice.

We also show complete 2A peptide-mediated ‘cleavage’ and stoichiometric production of two fluorescent proteins using a fluorescence resonance energy transfer-based system in multiple cell types including blood, thymus, spleen, bone marrow and early stem cell progenitors.

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